Article
Cloning and sequencing of Puumala virus Sotkamo strain S and M RNA segments: evidence for strain variation in hantaviruses and expression of the nucleocapsid protein.
The Journal of general virology - 1 Apr 1992
Vapalahti O, Kallio-Kokko H, Salonen E M, Brummer-Korvenkontio M, Vaheri A
Abstract excerpt
The prototype Puumala virus (PV) Sotkamo strain small (S) and medium (M) RNA genome segments were amplified by the polymerase chain reaction (PCR), cloned and sequenced. The S segment is 1830 nucleotides long with an open reading frame coding for 433 amino acids. The identity to the PV Hällnäs strain was 83% at the nucleotide and 96% at the amino acid level. The M segment in the Sotkamo strain is 3616 nucleotides...
Topics
- Amino Acid Sequence
- Animals
- Base Sequence
- Blotting, Western
- Cloning, Molecular
- Genetic Variation
- Orthohantavirus
- Hemorrhagic Fever with Renal Syndrome
- Molecular Sequence Data
- Polymerase Chain Reaction