Article
Sequences of wild Puumala virus genes show a correlation of genetic variation with geographic origin of the strains.
The Journal of general virology - 1 Feb 1994
Plyusnin A, Vapalahti O, Ulfves K, Lehväslaiho H, Apekina N, Gavrilovskaya I, Blinov V, Vaheri A
Abstract excerpt
An experimental scheme was developed for direct sequence analysis of Puumala virus-containing specimens from wild rodents (Clethrionomys glareolus). Total RNA isolated from rodent lung tissues was reverse-transcribed in the presence of a universal 11 nucleotide primer complementary to all three viral RNA segments followed by amplification in a PCR with gene-specific primers. A full-length PCR product of...
Topics
- Base Sequence
- Capsid
- Genes, Viral
- Genetic Variation
- Orthohantavirus
- Molecular Sequence Data
- Phylogeny
- Viral Core Proteins