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Article

Sequences of wild Puumala virus genes show a correlation of genetic variation with geographic origin of the strains.

The Journal of general virology - 1 Feb 1994

Plyusnin A, Vapalahti O, Ulfves K, Lehväslaiho H, Apekina N, Gavrilovskaya I, Blinov V, Vaheri A

Abstract excerpt

An experimental scheme was developed for direct sequence analysis of Puumala virus-containing specimens from wild rodents (Clethrionomys glareolus). Total RNA isolated from rodent lung tissues was reverse-transcribed in the presence of a universal 11 nucleotide primer complementary to all three viral RNA segments followed by amplification in a PCR with gene-specific primers. A full-length PCR product of...

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PMID: 8113763
DOI: 10.1099/0022-1317-75-2-405

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Sequences of wild Puumala virus genes show a correlation of genetic variation with geographic origin of the strains.PMID 8113763Read ↗

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