Article
Analysis of 6 VNTR loci by 'multiplex' PCR and automated fluorescent detection.
Human genetics - 1 Dec 1993
Tully G, Sullivan K M, Gill P
Abstract excerpt
The polymerase chain reaction was used to amplify six small variable number of tandem repeat loci in two reactions (D19S20 co-amplifying with D17S5 and D1S80; D17S766 co-amplifying with D16S83 and D17S24). When coupled with fluorescent detection of the products, this provides a rapid, highly discriminating automated test. Preferential amplification of small alleles, leading to 'allelic dropout' was found to occur...
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