Article
Manipulation of human B cells to confer immortality.
Human antibodies and hybridomas - 1 Oct 1992
Jonak Z L, Henri E, Trulli S, Jensen C P, Muirhead K A
Abstract excerpt
Electroporation was used to deliver genomic DNA from a lymphoid tumor to activated/stimulated human peripheral blood lymphocytes to create immortalized lymphoid cell lines. Activation of the recipient lymphocytes was essential for efficient immortalization. A panel of human B cell transfectant clones, each phenotypically representing specific stages of differentiation, resulted from the transfection. Monoclonal...
Topics
- Antibodies, Monoclonal
- B-Lymphocytes
- Cell Differentiation
- Cell Line, Transformed
- DNA
- Herpesvirus 4, Human
- Humans
- Lymphocyte Activation
- Oncogenes
- Phenotype
- Transfection