Article
A mutant lectin gene is rescued from an insertion element that blocks its expression.
The Plant cell - 1 Sept 1992
Okamuro J K, Goldberg R B
Abstract excerpt
The soybean lectin gene Le1 encodes a prevalent seed protein and is highly regulated during the life cycle. The mutant lectin gene allele le1 is not transcribed detectably, contains a 3.5-kb Tgm1 insertion element within its coding region 0.6 kb 3' to the transcription start site, and leads to a lectinless phenotype. To determine whether the Tgm1 element or a secondary mutation was responsible for repressing le1...
Topics
- Cloning, Molecular
- DNA Transposable Elements
- Gene Expression Regulation
- Lectins
- Mutation
- Plant Lectins
- Plants, Toxic
- Restriction Mapping
- Seeds
- Soybean Proteins
- Nicotiana
- Transcription, Genetic