Article
A P element chimera containing captured genomic sequences was recovered at the vestigial locus in Drosophila following targeted transposition.
Genetics - 1 Aug 1992
Heslip T R, Williams J A, Bell J B, Hodgetts R B
Abstract excerpt
A P element carrying the Dopa decarboxylase gene, P[Ddc], was targeted into vg21, a cryptic P element induced mutant allele of the vestigial (vg) locus. The resulting allele, vg28w, contained the expected P[Ddc] plus an additional 9.5 kb of DNA, captured from elsewhere on chromosome II. Reversion of the vg28w mutant allele demonstrated that the entire insert can excise but cannot reinsert at an appreciable...
Topics
- Alleles
- Animals
- Base Sequence
- Chimera
- DNA
- DNA Mutational Analysis
- DNA Transposable Elements
- Dopa Decarboxylase
- Drosophila melanogaster
- Female
- Male