Article
Localization of a chromosomal mutation affecting expression of extracellular lipase in Staphylococcus aureus.
Journal of bacteriology - 1 Jun 1992
Smeltzer M S, Gill S R, Iandolo J J
Abstract excerpt
We describe a Tn551 chromosomal insertion in Staphylococcus aureus S6C that results in sharply reduced expression of extracellular lipase. With Tn917 as a probe, the insertion in the original mutant (KSI905) was localized to a 12.6-kb EcoRI DNA fragment. The 12.6-kb fragment was cloned and used a...
Topics
- Base Sequence
- Blotting, Southern
- Chromosomes, Bacterial
- Cloning, Molecular
- DNA Transposable Elements
- Gene Expression Regulation, Bacterial
- Lipase
- Molecular Sequence Data
- Mutation
- Plasmids
- Restriction Mapping
- Staphylococcus aureus