Localization of a chromosomal mutation affecting expression of extracellular lipase in Staphylococcus aureus.

Abstract excerpt

We describe a Tn551 chromosomal insertion in Staphylococcus aureus S6C that results in sharply reduced expression of extracellular lipase. With Tn917 as a probe, the insertion in the original mutant (KSI905) was localized to a 12.6-kb EcoRI DNA fragment. The 12.6-kb fragment was cloned and used as a probe to identify a 26-kb EcoRI fragment containing the Tn551 insertion site in the S6C parent strain. Restriction...

Topics

Share this publication in a Topic to start or enrich a Post.

• Share to Base Sequence
• Share to Blotting, Southern
• Share to Cloning, Molecular