Article
[3Fe-4S] to [4Fe-4S] cluster conversion in Escherichia coli fumarate reductase by site-directed mutagenesis.
Biochemistry - 17 Mar 1992
Manodori A, Cecchini G, Schröder I, Gunsalus R P, Werth M T, Johnson M K
Abstract excerpt
Site-directed mutants of Escherichia coli fumarate reductase in which FrdB Cys204, Cys210, and Cys214 were individually replaced by Ser and in which Val207 was replaced by Cys were constructed and overexpressed in a strain of E. coli lacking a wild-type copy of fumarate reductase and succinate de...
Topics
- Amino Acid Sequence
- Catalysis
- Electron Spin Resonance Spectroscopy
- Escherichia coli
- Iron-Sulfur Proteins
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Mutation
- Oxidation-Reduction
- Plasmids
- Sequence Homology, Nucleic Acid
- Succinate Dehydrogenase