Article
Oxygen-evolving photosystem II preparation from wild type and photosystem II mutants of Synechocystis sp. PCC 6803.
Biochemistry - 25 Feb 1992
Kirilovsky D L, Boussac A G, van Mieghem F J, Ducruet J M, Sétif P R, Yu J J, Vermaas W F, Rutherford A W
Abstract excerpt
We present here a simple and rapid method which allows relatively large quantities of oxygen-evolving photosystem II- (PS-II-) enriched particles to be obtained from wild-type and mutants of the cyanobacterium Synechocystis 6803. This method is based on that of Burnap et al. [Burnap, R., Koike, H., Sotiropoulou, G., Sherman, L. A., & Inoue, Y. (1989) Photosynth. Res. 22, 123-130] but is modified so that the whole...
Topics
- Chlorophyll
- Chlorophyll A
- Cyanobacteria
- Electron Spin Resonance Spectroscopy
- Electrophoresis, Polyacrylamide Gel
- Fluorescence Polarization
- Genes, Bacterial
- Intracellular Membranes
- Luminescent Measurements
- Mutation
