Article
Regulation of a cytochrome c2 isoform in wild-type and cytochrome c2 mutant strains of Rhodobacter sphaeroides.
Archives of biochemistry and biophysics - 1 Feb 1992
Rott M A, Fitch J, Meyer T E, Donohue T J
Abstract excerpt
In Rhodobacter sphaeroides, mutations that suppress the photosynthetic deficiency (spd mutations) of strains lacking cytochrome c2 (cyt c2) cause accumulation of a periplasmic cyt c2 isoform that has been designated isocytochrome c2 (isocyt c2). In this study, a new method for purification of both cyt c2 and isocyt c2 is described that uses periplasmic fluid as a starting material. In addition, antiserum to...
Topics
- Cytochrome c Group
- Cytochromes c2
- Electrophoresis, Polyacrylamide Gel
- Genotype
- Immunoblotting
- Isoenzymes
- Kinetics
- Molecular Weight
- Mutation
- Rhodobacter sphaeroides