Article
[Baculovirus p74 gene is a species-specific gene].
Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica - 1 Sept 2003
Wu Wu-Wei, Wang Jing-Wen, Xie Fei, Long Qing-Xin, Wang Xun-Zhang
Abstract excerpt
The p74 gene of Autographa californica multicasid nucleopolyhedrovirus (AcMNPV) bacmid was knockouted and substituted by the p74 gene of Spodoptera litura multicapsid nucleopolyhedrovirus (SpltMNPV), using RecA-mediated homologous recombination in the E. coli. No selection marker, which might influence the expression and function of p74 gene, was left in the modified p74 locus. The promoter of AcMNPV p74 gene...
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