Article
Phosphorylation of the potyvirus capsid protein by protein kinase CK2 and its relevance for virus infection.
The Plant cell - 1 Sept 2003
Ivanov Konstantin I, Puustinen Pietri, Gabrenaite Rasa, Vihinen Helena, Rönnstrand Lars, Valmu Leena, Kalkkinen Nisse, Mäkinen Kristiina
Abstract excerpt
We reported previously that the capsid protein (CP) of Potato virus A (PVA) is phosphorylated both in virus-infected plants and in vitro. In this study, an enzyme that phosphorylates PVA CP was identified as the protein kinase CK2. The alpha-catalytic subunit of CK2 (CK2alpha) was purified from tobacco and characterized using in-gel kinase assays and liquid chromatography-tandem mass spectrometry. The tobacco...
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