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Article

Protocol for the sequence analysis of ryanodine receptor subtype 1 gene transcripts from human leukocytes.

Anesthesiology - 1 Aug 2003

Kraev Natasha, Loke Julian C P, Kraev Alexander, MacLennan David H

Abstract excerpt

BACKGROUND: The search for novel mutations in the ryanodine receptor subtype 1 (RYR1) gene causing malignant hyperthermia and central core disease is hampered by the fact that the gene contains 106 exons. Searching for novel mutations in complementary DNA (cDNA) requires an invasive muscle biopsy. Accordingly, an alternate source of RYR1 cDNA was sought for sequence analysis. METHODS: Leukocytes were isolated...

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Topics

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Share to Cloning, Molecular
Share to DNA, Complementary
Share to DNA Primers

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Share to Leukocytes

Share to Muscle, Skeletal

Share to Reverse Transcriptase Polymerase Chain Reaction

Share to Ryanodine Receptor Calcium Release Channel

Share to Transcription, Genetic

Source

Identifiers

PMID: 12883401
DOI: 10.1097/00000542-200308000-00010

Genes

RYR1

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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Protocol for the sequence analysis of ryanodine receptor subtype 1 gene transcripts from human leukocytes.PMID 12883401Read ↗

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