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Article

Direct Amplification of rRNA Genes in Diagnosis of Bacterial Infections

1 Jan 2000

Abstract excerpt

A broad-range bacterial PCR targeting rRNA genes (rDNAs) was used to directly analyze 536 clinical samples obtained from 459 hospitalized patients during a 4-year study period. The molecular diagnosis based on DNA sequencing of the PCR product was compared to that obtained by bacterial culture. The bacteriological diagnosis was concordant for 447 (83%) specimens. Broad-range rDNA PCR was the only method that...

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Identifiers

PMID: 10618059
DOI: 10.1128/jcm.38.1.32-39.2000

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