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Article

In vitro development and characterization of a manatee bronchial cell line.

In vitro cellular & developmental biology. Animal - 1 Jan 2000

Sweat James M, Johnson Calvin M, Gibbs E Paul J

Abstract excerpt

In the present study, culture conditions that promote the growth and differentiation of manatee respiratory tract epithelial cells toward a mucociliary phenotype were determined. Characterization of a manatee-specific cell line enables investigators to conduct in vitro testing where live-animal experimentation is not possible. Cell cultures were established from both explants and enzymatically dissociated cells...

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PMID: 12841797
DOI: 10.1290/1543-706X(2003)039<0249:IVDACO>2.0.CO;2

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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