Article
Characterization of normal breast epithelial cells in primary cultures: differentiation and growth factor receptors studies.
In vitro cellular & developmental biology : journal of the Tissue Culture Association - 1 Jan 1992
Berthon P, Pancino G, de Cremoux P, Roseto A, Gespach C, Calvo F
Abstract excerpt
The growth and differentiation of normal human mammary epithelial cells (HMEC) were studied after propagation of serial cultures from breast tissue biopsies from 42 mammoplasty patients. Cells were grown for up to 7 mo. in low calcium medium. HMEC cultures displayed heterogeneous growth patterns, according to the average doubling time of 44 +/- 6 h for 32 generations. Proliferation peaked at Day 30. HMEC...
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