Article
Infection with a plasmid-free variant Chlamydia related to Chlamydia trachomatis identified by using multiple assays for nucleic acid detection.
Journal of clinical microbiology - 1 Nov 1992
An Q, Radcliffe G, Vassallo R, Buxton D, O'Brien W J, Pelletier D A, Weisburg W G, Klinger J D, Olive D M
Abstract excerpt
Clinical samples in transport media from 40 patients exhibiting pathologies potentially caused by Chlamydia trachomatis infection were analyzed for chlamydial nucleic acid, and the results were compared with those of culture. Chlamydial culture was performed by a shell vial centrifugation method with HeLa 229 host cells. Polymerase chain reaction (PCR) assays were used to detect either regions on a 7.5-kb plasmid...
Topics
- Animals
- Base Sequence
- Cattle
- Chlamydia Infections
- Chlamydia trachomatis
- DNA Probes
- DNA, Bacterial
- Evaluation Studies as Topic
- Female
- Genetic Techniques