Article
Cultivation-dependent and -independent approaches for determining bacterial diversity in heavy-metal-contaminated soil.
Applied and environmental microbiology - 1 Jun 2003
Ellis Richard J, Morgan Philip, Weightman Andrew J, Fry John C
Abstract excerpt
In recent years, culture-independent methods have been used in preference to traditional isolation techniques for microbial community analysis. However, it is questionable whether uncultured organisms from a given sample are important for determining the impact of anthropogenic stress on indigenous communities. To investigate this, soil samples were taken from a site with patchy metal contamination, and the...
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