Article
Unusual nucleotide-binding properties of the chloroplast protein import receptor, atToc33.
FEBS letters - 5 Jun 2003
Aronsson Henrik, Combe Jonathan, Jarvis Paul
Abstract excerpt
Arabidopsis Toc33 (atToc33) is a GTP-binding protein of the chloroplast outer envelope membrane. We studied its nucleotide-binding properties in vitro, and found that it binds GTP, GDP and XTP, with similar efficiencies, but not ATP. We further demonstrated that atToc33 has intrinsic GTPase activity. Mutations within the putative G4 motif of the atToc33 nucleotide-binding domain (D217N, D219N and E220Q) had no...
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