Article
Quantitation of mecA transcription in oxacillin-resistant Staphylococcus aureus clinical isolates.
Journal of bacteriology - 1 Jun 2003
Rosato Adriana E, Craig William A, Archer Gordon L
Abstract excerpt
The transcription of mecA, the gene required for oxacillin resistance in staphylococci, was quantified in a collection of 65 geographically and genetically diverse clinical and 8 defined laboratory Staphylococcus aureus isolates. mecA transcription was measured by real-time reverse transcription-PCR, confirmed by Northern blot analysis, and correlated with the presence and DNA sequence of the two mecA repressors,...
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