Article
Natural disulfide bond-disrupted mutants of AVR4 of the tomato pathogen Cladosporium fulvum are sensitive to proteolysis, circumvent Cf-4-mediated resistance, but retain their chitin binding ability.
The Journal of biological chemistry - 25 Jul 2003
van den Burg Harrold A, Westerink Nienke, Francoijs Kees-Jan, Roth Ronelle, Woestenenk Esmeralda, Boeren Sjef, de Wit Pierre J G M, Joosten Matthieu H A J, Vervoort Jacques
Abstract excerpt
The extracellular AVR4 elicitor of the pathogenic fungus Cladosporium fulvum induces defense responses in the tomato genotype Cf-4. Here, the four disulfide bonds of AVR4 were identified as Cys-11-41, Cys-21-27, Cys-35-80, and Cys-57-72 by partial reduction with Tris-(2-carboxyethyl)-phosphine hydrochloride, subsequent cyanylation, and base-catalyzed chain cleavage. The resulting peptide fragments were analyzed...
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