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Article

Gene expression regulating epithelial intercellular junction biogenesis during human blastocyst development in vitro

1 May 2003

Abstract excerpt

We investigated gene expression associated with trophectoderm epithelial intercellular junction formation in single human embryos at different stages of cleavage using RT-PCR methods based upon magnetic bead separation of polyA+ RNA. Trophectoderm tight junction (TJ) and desmosome biogenesis contribute to intercellular sealing and tissue integrity, critical for vectorial transport and blastocoel cavity formation....

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PMID: 12728017
DOI: 10.1093/molehr/gag033

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