Article
Expression of short-chain acyl-CoA dehydrogenase (SCAD) proteins in the liver of SCAD deficient mice after hydrodynamic gene transfer.
Molecular genetics and metabolism - 1 Apr 2003
Holm David A, Dagnaes-Hansen Frederik, Simonsen Henrik, Gregersen Niels, Bolund Lars, Jensen Thomas G, Corydon Thomas J
Abstract excerpt
Hydrodynamic administration of naked DNA was investigated as a method for in vivo expression of variant proteins involved in metabolic diseases, using short-chain acyl-CoA dehydrogenase (SCAD) deficient mice (BALB/cByJ) as a model. Human SCAD wild-type (WT) and two disease-associated SCAD variant proteins (R147W and G185S) were expressed in mouse liver by means of single injections of SCAD cDNA under the control...
Topics
- Animals
- Blotting, Western
- Butyryl-CoA Dehydrogenase
- DNA
- DNA, Complementary
- Gene Transfer Techniques
- Genetic Variation
- Green Fluorescent Proteins
- Humans
- Liver
- Liver Extracts
- Luminescent Proteins
- Mice