Article
Molecular characterization of rice hsp101: complementation of yeast hsp104 mutation by disaggregation of protein granules and differential expression in indica and japonica rice types.
Plant molecular biology - 1 Mar 2003
Agarwal Manu, Sahi Chandan, Katiyar-Agarwal Surekha, Agarwal Sangeeta, Young Todd, Gallie Daniel R, Sharma Vishva Mitra, Ganesan K, Grover Anil
Abstract excerpt
HSP100 protein is an important component of the heat-shock response in diverse organisms. Using specific primers based on cDNA sequence, rice hsp101 gene was PCR-amplified and sequenced. Southern analysis revealed that there appears to be a single gene per haploid genome coding for HSP101 protein in rice. Northern analysis showed that expression of hsp101 transcript is strictly heat-inducible and induction is...
Topics
- Arsenites
- Blotting, Southern
- Blotting, Western
- Cell Division
- Cloning, Molecular
- DNA, Plant
- Gene Expression Regulation, Plant
- Genes, Plant
- Genetic Complementation Test