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A Novel Method for Generating Xeno-Free Human Feeder Cells for Human Embryonic Stem Cell Culture

1 Jun 2008

Abstract excerpt

Long-term cultures of human embryonic stem (hES) cells require a feeder layer for maintaining cells in an undifferentiated state and increasing karyotype stability. In routine hES cell culture, mouse embryonic fibroblast (MEF) feeders and animal component-containing media (FBS or serum replacement) are commonly used. However, the use of animal materials increases the risk of transmitting pathogens to hES cells...

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PMID: 18513158
DOI: 10.1089/scd.2007.0236

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