Article
Development and evaluation of genotypic assays for the detection and characterization of enterotoxigenic Escherichia coli.
Diagnostic microbiology and infectious disease - 1 Feb 2003
Steinsland Hans, Valentiner-Branth Palle, Grewal Harleen M S, Gaastra Wim, Mølbak K Kåre, Sommerfelt Halvor
Abstract excerpt
We developed and evaluated a method to genotypically identify enterotoxigenic Escherichia coli (ETEC) and to characterize these organisms with respect to 18 of 21 known colonization factors (CFs). The method, which is based on polynucleotide DNA-DNA colony hybridization, includes a pooled toxin probe assay to identify ETEC, and individual probe assays to detect the enterotoxins STp, STh, and LT, and the CFs...
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