Article
ESTs as a source for sequence polymorphism discovery in sugarcane: example of the Adh genes.
TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik - 1 Jan 2003
Grivet L, Glaszmann J-C, Vincentz M, da Silva F, Arruda P
Abstract excerpt
Expressed sequence tags (ESTs) have proven to be a valuable tool to discover single nucleotide polymorphism (SNP) in human genes but their use for this purpose is still limited in higher plants. Using a database of approximately 250,000 sugarcane ESTs we have recovered 219 sequences encoding alcohol dehydrogenases ( Adh), which tagged 178 distinct cDNAs from 27 libraries, constructed from at least four different...
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