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FREQUENCY OF MULTIPLE INSEMINATIONS IN FIELD-COLLECTED ANOPHELES GAMBIAE FEMALES REVEALED BY DNA ANALYSIS OF TRANSFERRED SPERM

1 Jan 2003

Abstract excerpt

We investigated the frequencies of single and multiple matings in field-collected female Anopheles gambiae by conducting microsatellite DNA analyses on the sperm contained within their spermatheca. Amplifcation by a polymerase chain reaction (PCR) at four loci allowed the detection of sperm extracts exhibiting more than two alleles per locus, thereby revealing the occurrence of multiple inseminations. Polyandry...

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PMID: 12556139
DOI: 10.4269/ajtmh.2003.68.1.0680001

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