Article
Single-strand conformation polymorphism (SSCP) assays for major histocompatibility complex B genotyping in chickens.
Poultry science - 1 Dec 2002
Goto R M, Afanassieff M, Ha J, Iglesias G M, Ewald S J, Briles W E, Miller M M
Abstract excerpt
We have developed a DNA-based method for defining MHC B system genotypes in chickens. Genotyping by this method requires neither prior determination of allele-specific differences in nucleotide sequence nor the preparation of haplotype-specific alloantisera. Allelic differences at chicken B-F (class I) and B-L (class II) loci are detected in PCR single-strand conformation polymorphism (SSCP) assays. PCR primer...
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