Article
The Glc7p-interacting protein Bud14p attenuates polarized growth, pheromone response, and filamentous growth in Saccharomyces cerevisiae.
Eukaryotic cell - 1 Dec 2002
Cullen Paul J, Sprague George F
Abstract excerpt
A genetic selection in Saccharomyces cerevisiae for mutants that stimulate the mating pathway uncovered a mutant that had a hyperactive pheromone response pathway and also had hyperpolarized growth. Cloning and segregation analysis demonstrated that BUD14 was the affected gene. Disruption of BUD14 in wild-type cells caused mild stimulation of pheromone response pathway reporters, an increase in sensitivity to...
Topics
- Cell Division
- DNA Mutational Analysis
- Fluorescent Antibody Technique, Indirect
- Genotype
- Green Fluorescent Proteins
- Intracellular Signaling Peptides and Proteins
- Luminescent Proteins
- MAP Kinase Kinase Kinases
- Mating Factor
- Microfilament Proteins
- Mutation
- Peptides
- Phosphoprotein Phosphatases