Article
The temperature dependence of activity and structure for the most prevalent mutant aldolase B associated with hereditary fructose intolerance.
Archives of biochemistry and biophysics - 15 Dec 2002
Malay Ali D, Procious Sheri L, Tolan Dean R
Abstract excerpt
Hereditary fructose intolerance (HFI) is an autosomal recessive disorder in humans which is caused by mutations in the aldolase B gene. The most common HFI allele encodes an enzyme with an A149P substitution (AP-aldolase). A lysis method suitable for aggregation-prone proteins overexpressed in bacteria was developed. The enzyme's structure and function is investigated as a function of temperature. Near-UV CD...
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