Article
Quantitative analysis of ITS2 sequences in trichostrongyle parasites.
International journal for parasitology - 1 Nov 2002
von Samson-Himmelstjerna G, Harder A, Schnieder T
Abstract excerpt
Real-time PCR assays were developed to identify and quantify common gastrointestinal nematodes of ruminants. The assays were based on genus-specific primer and probe combinations derived from the second internal transcribed spacer (ITS2) ribosomal DNA transcription unit of Haemonchus contortus, Ostertagia leptospicularis, Trichostrongylus colubriformis and Cooperia curticei. The TaqMan probes for the first two...
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