Article
Isolation and characterization of the glnD gene of Gluconacetobacter diazotrophicus, encoding a putative uridylyltransferase/uridylyl-removing enzyme.
Gene - 4 Sept 2002
Perlova Olena, Nawroth Roman, Zellermann Eva-Maria, Meletzus Dietmar
Abstract excerpt
The glnD gene of Gluconacetobacter diazotrophicus was isolated by complementation of the Azotobacter vinelandii glnD (nfrX) mutant strain MV17 using a pLAFR3 cosmid library. The 5 kb chromosomal DNA region encoding the glnD gene on cosmid pAD401 was identified by introduction of deletions as well as subcloning of restriction fragments followed by subsequent DNA sequencing. Three open reading frames were...
Topics
- Acetobacteraceae
- Amino Acid Sequence
- Base Sequence
- Blotting, Western
- Chromosome Mapping
- Cloning, Molecular
- DNA, Bacterial
- Gene Expression Regulation, Bacterial
- Gene Expression Regulation, Enzymologic
- Molecular Sequence Data
- Mutagenesis, Insertional
- Mutation