Article
Denaturing gradient gel electrophoretic multilocus sequence typing of Staphylococcus aureus isolates.
Electrophoresis - 1 Sept 2002
Gürtler Volker, Barrie Helen D, Mayall Barrie C
Abstract excerpt
To obviate the need for multilocus sequencing, a method using denaturing gradient gel electrophoresis (DGGE) was developed for the multilocus sequence typing (MLST) of Staphylococcus aureus isolates. Sequence types (STs) were obtained on the basis of sequences of polymerase chain reaction (PCR) products from seven housekeeping genes and compared to the reference MLST database. The melt curves, sequences and DGGE...
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