Article
Nuclear DNA PCR-RFLPs that distinguish African and European honey bee groups of subspecies. I: Comparison of long PCR and standard PCR to screen for polymorphisms.
Biochemical genetics - 1 Aug 2002
Suazo Alonso, Hall H Glenn
Abstract excerpt
Nuclear DNA RFLPs betweenAfrican and European honeybees (Apis mellifera L.) were sought by amplifying short (1-2 kbp) and long (> 5 kbp) anonymous regions of DNA and digesting the respective PCR products with a collection of restriction enzymes. Three short and three long regions were each screened with 26-31 enzymes. From a total of 163 locus enzyme combinations (LECs), seven revealed informative polymorphisms....
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