Article
Comparative assessment of genotyping methods for epidemiologic study of Burkholderia cepacia genomovar III.
Journal of clinical microbiology - 1 Sept 2002
Coenye Tom, Spilker Theodore, Martin Alissa, LiPuma John J
Abstract excerpt
We analyzed a collection of 97 well-characterized Burkholderia cepacia genomovar III isolates to evaluate multiple genomic typing systems, including pulsed-field gel electrophoresis (PFGE), BOX-PCR fingerprinting and random amplified polymorphic DNA (RAPD) typing. The typeability, reproducibility, and discriminatory power of these techniques were evaluated, and the results were compared to each other and to data...
Topics
- Bacterial Typing Techniques
- Burkholderia Infections
- Burkholderia cepacia
- Cystic Fibrosis
- Electrophoresis, Gel, Pulsed-Field
- Genotype
- Humans
- Molecular Epidemiology
- Polymerase Chain Reaction
- Random Amplified Polymorphic DNA Technique
