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Cytogenetic analysis of giant oocytes and zygotes to assess their relevance for the development of digynic triploidy

29 Aug 2002

Abstract excerpt

BACKGROUND: In order to assess the role of binucleate giant oocytes for generating digynic triploidy, we studied their frequency, maturation patterns and chromosomal complements at metaphase II (MII) or after fertilization. METHODS: Uncleaved, giant zygotes were incubated with podophyllotoxin and vinblastine, treated with hypotonic solution and fixed by a gradual fixation method. Giant MII oocytes were directly...

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PMID: 12202429
DOI: 10.1093/humrep/17.9.2388

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