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Article

Cloning and characterization of a divergent alpha-tubulin that is expressed specifically in dividing amebae of Naegleria gruberi.

Gene - 26 Jun 2002

Chung Sunglan, Cho Jaeyoung, Cheon HyeonJoo, Paik Soonyoung, Lee JooHun

Abstract excerpt

A novel alpha-tubulin gene (alpha6) was cloned from a genomic library of Naegleria gruberi strain NB-1 and characterized. The open reading frame of alpha6 contained 1359 nucleotides encoding a protein of 452 amino acids (aa) with a calculated molecular weight of 50.5 kDa. The nucleotide sequence of the open reading frame of alpha6 showed considerable divergence (68.4% identity) when compared with previously...

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PMID: 12137945
DOI: 10.1016/s0378-1119(02)00509-7

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