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Article

Combined detection and genotyping of Chikungunya virus by a specific reverse transcription-polymerase chain reaction.

Journal of medical virology - 1 Jul 2002

Hasebe F, Parquet M C, Pandey B D, Mathenge E G M, Morita K, Balasubramaniam V, Saat Z, Yusop A, Sinniah M, Natkunam S, Igarashi A

Abstract excerpt

A reverse transcription-polymerase chain reaction (RT-PCR) was developed for the detection of Chikungunya virus infection. Based on the nonstructural protein 1 (nsP1) and glycoprotein E1 (E1) genes of Chikungunya, two primer sets were designed. Total RNA were extracted from the cell culture fluid of Aedes albopictus C6/36 cells inoculated with the S27 prototype virus, isolated in Tanzania in 1953, and the...

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PMID: 12116030
DOI: 10.1002/jmv.10085

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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