Use of PCR-RFLP for genotyping 16S rRNA and characterizing bacteria cultured from halibut fry.

doi:10.1139/w02-033

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Article

Use of PCR-RFLP for genotyping 16S rRNA and characterizing bacteria cultured from halibut fry.

Canadian journal of microbiology - 1 May 2002

Jensen Sigmund, Bergh Oivind, Enger Oivind, Hjeltnes Brit

Abstract excerpt

Small subunit ribosomal genes were explored using PCR-RFLP to facilitate the characterization of bacteria cultured from reared fry of the Atlantic halibut (Hippoglossus hippoglossus). Concern has been expressed about pathogen invasion in larvae lacking a counteracting normal flora that may aid the immune system in producing robust noninfected individuals. In this study, pure cultured representatives of normal...

Topics

Animals
Bacterial Typing Techniques
Culture Media
DNA, Ribosomal
Flounder
Gammaproteobacteria
Genotype
Larva
Phylogeny
Polymerase Chain Reaction
Polymorphism, Restriction Fragment Length
RNA, Ribosomal, 16S
Sequence Analysis, DNA

Source

Open in PubMed

Identifiers

PMID: 12109877
DOI: 10.1139/w02-033

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