Article
[Application of heteroduplex mobility assay in genetic subtyping on human immunodeficiency virus type 1].
Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi - 1 Apr 2002
Chen Liang, Yan Yansheng, Weng Yuwei, Wang Huirong, Wu Shouli, Chen Ge, Zheng Zhaoshuang, Zheng Jian, Yan Pingping
Abstract excerpt
OBJECTIVE: Using heteroduplex mobility assay (HMA) to subtype human immunodeficiency virus type 1 (HIV-1) for the purpose of understanding HIV-1 subtype epidemic in Fujian province. METHODS: DNA fragments of HIV-1 env gene were amplified from peripheral blood mononuclear cell (PBMC) cocultures of HIV-1 infected individuals by nested polymerase chain reaction (PCR). Heteroduplexs were formed through hybridizing...
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