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Simplified Strategy for Detection of Recombinant Human Immunodeficiency Virus Type 1 Group M Isolates by <i>gag/env</i> Heteroduplex Mobility Assay

1 Jan 2000

Abstract excerpt

We developed a heteroduplex mobility assay in the gag gene (gag HMA) for the identification of group M subtypes A to H. The assay covers the region coding for amino acid 132 of p24 to amino acid 20 of p7 (according to human immunodeficiency virus type 1 [HIV-1] ELI, 460 bp). The gag HMA was compared with sequencing and phylogenetic analysis of an evaluation panel of 79 HIV-1 group M isolates isolated from...

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Identifiers

PMID: 10590125
DOI: 10.1128/jvi.74.1.363-370.2000

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