Article
Detection of a primer-binding site polymorphism for the STR locus D16S539 using the Powerplex 1.1 system and validation of a degenerate primer to correct for the polymorphism.
Journal of forensic sciences - 1 Mar 2002
Nelson Mark S, Levedakou Eleni N, Matthews Janis R, Early Buddy E, Freeman David A, Kuhn Cynthia A, Sprecher Cynthia J, Amin Ashima S, McElfresh Kevin C, Schumm James W
Abstract excerpt
Quality assurance samples submitted from the NCSBI as part of a contract with TBTG to outsource DNA Database samples showed unexpected discrepancies for the locus D16S539 when all other loci yielded identical results. Discrepancies observed included allele drop out and an imbalance in sister alleles with samples returned from TBTG. This led to a comprehensive review of the technical procedures used between the...
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