Article
Development and evaluation of a DNA enzyme immunoassay method for env genotyping of subtypes A through G of human immunodeficiency virus type 1 group M, with discrimination of the circulating recombinant forms CRF01_AE and CRF02_AG.
Journal of clinical microbiology - 1 Mar 2002
Plantier Jean-Christophe, Vergne Laurence, Damond Florence, MBoup Souleymane, MPoudi-NGole Eitel, Buzelay Laurence, Farfara Isabelle, Brand Denys, Peeters Martine, Brun-Vézinet Françoise, Delaporte Eric, Barin Francis
Abstract excerpt
The tools currently available for genetic subtyping of human immunodeficiency virus type 1 are laborious or can be used only for the analysis of a limited number of samples and/or subtypes. We developed and evaluated a molecular biology-based method using subtype-specific oligonucleotide probes for env genotyping of subtypes A through G, CRF01_AE, and CRF02_AG. DNA enzyme immunoassay (DEIA) genotyping is based on...
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