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Measuring tubulin content in <i>Toxoplasma gondii</i> : A comparison of laser-scanning confocal and wide-field fluorescence microscopy

5 Feb 2002

Abstract excerpt

Toxoplasma gondii is an intracellular parasite that proliferates within most nucleated cells, an important human pathogen, and a model for the study of human and veterinary parasitic infections. We used a stable yellow fluorescent protein-alpha-tubulin transgenic line to determine the structure of the microtubule cytoskeleton in T. gondii. Imaging of living yellow fluorescent protein-alpha-tubulin parasites by...

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PMID: 11830634
DOI: 10.1073/pnas.022554999

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