Article
mbgA-dependent lactose utilization by Bacillus megaterium.
Current microbiology - 1 Feb 2002
Shaw Gwo-Chyuan, Chiou Chih-Yung, Chou Yi-Hua, Li Jen-Ming
Abstract excerpt
The beta-galactosidase-encoding mbgA gene has recently been cloned from Bacillus megaterium. We now report that disruption of the chromosomal mbgA rendered B. megaterium unable to utilize lactose as a sole carbon source. Complementation of the mbgA mutant with a multicopy plasmid carrying intact mbga restored the ability to utilize lactose for cell growth. Crude extracts from the wild-type B. megaterium cells...
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