Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Active site of deblocking aminopeptidase from Pyrococcus horikoshii.

Biochemical and biophysical research communications - 25 Jan 2002

Onoe Shinji, Ando Susumu, Ataka Mitsuo, Ishikawa Kazuhiko

Abstract excerpt

New hyperthermostable aminopeptidase from the hyperthermophilic archaeon Pyrococcus horikoshii has acylamino acid releasing (deblocking) activity for acyl (blocked) peptides. Such an enzyme can be used for N-terminal sequencing of acyl peptides. To clarify the active site of the deblocking aminopeptidase, we prepared three mutants in which one of the three possible active site amino acid residues (Asp or Glu) was...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Amino Acid Sequence
Share to Aminopeptidases
Share to Binding Sites

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Catalytic Domain

Share to Cobalt

Share to Dose-Response Relationship, Drug

Share to Kinetics

Share to Sequence Homology, Amino Acid

Source

Identifiers

PMID: 11798173
DOI: 10.1006/bbrc.2001.6327

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Active site of deblocking aminopeptidase from Pyrococcus horikoshii.PMID 11798173Read ↗

Novel bifunctional hyperthermostable carboxypeptidase/aminoacylase from Pyrococcus horikoshii OT3.Semantic proximity Read ↗

The mechanism of autocatalytic activation of plant-type L-asparaginases.Semantic proximity Read ↗

Chemical rescue of the post-translationally carboxylated lysine mutant of allantoinase and dihydroorotase by metal ions and short-chain carboxylic acids.Semantic proximity Read ↗

Hydrolytic reaction by zinc finger mutant peptides: successful redesign of structural zinc sites into catalytic zinc sites.Semantic proximity Read ↗

Characterization and preliminary mutation analysis of a thermostable alanine racemase from Thermoanaerobacter tengcongensis MB4.Semantic proximity Read ↗

Thermostability reinforcement through a combination of thermostability-related mutations of N-carbamyl-D-amino acid amidohydrolase.Semantic proximity Read ↗

Improving the catalytic activity of hyperthermophilic Pyrococcus horikoshii prolidase for detoxification of organophosphorus nerve agents over a broad range of temperatures.Semantic proximity Read ↗

Structural basis for catalysis and substrate specificity of Agrobacterium radiobacter N-carbamoyl-D-amino acid amidohydrolase.Semantic proximity Read ↗

Structural role of a conserved active site cis proline in the Thermotoga maritima acetyl esterase from the carbohydrate esterase family 7.Semantic proximity Read ↗

Functional similarities of a thermostable protein-disulfide oxidoreductase identified in the archaeon Pyrococcus horikoshii to bacterial DsbA enzymes.Semantic proximity Read ↗

Structure-based modification of D-alanine-D-alanine ligase from Thermotoga maritima ATCC 43589 for depsipeptide synthesis.Semantic proximity Read ↗

Analysis of the putative substrate binding region of hyperthermophilic endoglucanase from Pyrococcus horikoshii.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.