Article
Genotyping shorthorn cattle for generalised glycogenosis.
Australian veterinary journal - 1 Nov 2001
Dennis J A, Healy P J
Abstract excerpt
OBJECTIVE: To develop a procedure for routine genotyping of Shorthorn cattle for the generalised glycogenosis allele in exon 18 of the acidic alpha-glucosidase gene. PROCEDURE: Allele-specific amplification and double mismatch amplification procedures for the discrimination of the exon 18 alleles were evaluated using leucocytes and hair roots as sources of target DNA. RESULTS: Allele-specific amplification was...
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