Genotyping shorthorn cattle for generalised glycogenosis.

doi:10.1111/j.1751-0813.2001.tb10897.x

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Article

Genotyping shorthorn cattle for generalised glycogenosis.

Australian veterinary journal - 1 Nov 2001

Dennis J A, Healy P J

Abstract excerpt

OBJECTIVE: To develop a procedure for routine genotyping of Shorthorn cattle for the generalised glycogenosis allele in exon 18 of the acidic alpha-glucosidase gene. PROCEDURE: Allele-specific amplification and double mismatch amplification procedures for the discrimination of the exon 18 alleles were evaluated using leucocytes and hair roots as sources of target DNA. RESULTS: Allele-specific amplification was...

Topics

Alleles
Animals
Breeding
Cattle
Cattle Diseases
DNA Primers
Electrophoresis, Agar Gel
Female
Genotype
Glycogen Storage Disease
Glycogen Storage Disease Type II
Hair Follicle
Leukocytes
Male
Polymerase Chain Reaction
Sensitivity and Specificity
alpha-Glucosidases

Source

Open in PubMed

Identifiers

PMID: 11789914
DOI: 10.1111/j.1751-0813.2001.tb10897.x

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