Article
Insertional inactivation of the menG gene, encoding 2-phytyl-1,4-naphthoquinone methyltransferase of Synechocystis sp. PCC 6803, results in the incorporation of 2-phytyl-1,4-naphthoquinone into the A(1) site and alteration of the equilibrium constant between A(1) and F(X) in photosystem I.
Biochemistry - 8 Jan 2002
Sakuragi Yumiko, Zybailov Boris, Shen Gaozhong, Jones A Daniel, Chitnis Parag R, van der Est Art, Bittl Robert, Zech Stephan, Stehlik Dietmar, Golbeck John H, Bryant Donald A
Abstract excerpt
A gene encoding a methyltransferase (menG) was identified in Synechocystis sp. PCC 6803 as responsible for transferring the methyl group to 2-phytyl-1,4-naphthoquinone in the biosynthetic pathway of phylloquinone, the secondary electron acceptor in photosystem I (PS I). Mass spectrometric measurements showed that targeted inactivation of the menG gene prevented the methylation step in the synthesis of...
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