Article
Cysteine-reactive fluorescence probes of catalytic sites of ATP synthase.
Archives of biochemistry and biophysics - 1 Jan 2002
Weber Joachim, Bijol Vanesa, Wilke-Mounts Susan, Senior Alan E
Abstract excerpt
We searched for new fluorescent probes of catalytic-site nucleotide binding in F(1)F(0)-ATP synthase by introducing Cys mutations at positions in or close to catalytic sites and then reacting Cys-mutant F(1) with thiol-reactive fluorescent probes. Four suitable mutant/probe combinations were identified. beta F410C labeled by 7-fluorobenz-2-oxa-1,3-diazole-4-sulfonamide (ABD-F) gave very large signal changes in...
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